Stimulation of cellular longevity using crispr-cas9 in aging-associated genes

María Fernanda Latorre Barragán; Freddy Raúl García Cárdenas; Marco Vinicio Culqui Sánchez

doi:10.59471/ijhsc202498

Authors

María Fernanda Latorre Barragán Universidad Regional Autónoma de Los Andes, Matriz Ambato, Ecuador Author https://orcid.org/0000-0002-9280-705X
Freddy Raúl García Cárdenas Universidad Regional Autónoma de Los Andes, Matriz Ambato, Ecuador Author https://orcid.org/0000-0002-1328-6322
Marco Vinicio Culqui Sánchez Universidad Regional Autónoma de Los Andes, Matriz Ambato, Ecuador Author https://orcid.org/0000-0002-8957-1752

DOI:

https://doi.org/10.59471/ijhsc202498

Keywords:

Cell senescence, cell longevity, KLOTHO, FOXO, genes , Telomers, CRISPR-CAS9

Abstract

Cellular senescence is a natural process of all living organisms. The genetic bases of this process have allowed the recognition of genes whose expression has been related to longevity and cell aging. The genes KLOTHO, FOXO, and those associated with telomere maintenance are three examples of these. The development of gene editing techniques is being directed at genes of biomedical interest such as those mentioned above. This literature review presents discoveries made on the genetic manipulation of the KLOTHO, FOXO and genes associated with telomere maintenance using the CRISPR-CAS9 tool. As a result, it is reported that the reduction of the expression of these genes has resulted in the increase of processes that lead to rapid aging. On the contrary, its overexpression increases the maintenance of the chemical and biological balance in organisms both in vivo and in vitro. The development of the precision of advanced gene manipulation techniques seeks not only the study of new biochemical pathways but also possible applications in medicine focused on supporting the well-being of living organisms.

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